Methods and applications of semi-permeable capsules with current application to high-throughput single cell multi-omics

The focus of this proposal is the development of capsules, a technological platform with applications to isolation, growth, sorting, dialysis and genomic analysis of single cells or molecules. The platform addresses multiple needs ranging from measurement and diagnostics, screening, and potentially cell-based therapy. We recently developed a method to produce capsules robustly, with a novel composition that satisfies design requirements for these applications. A report-of-invention has been filed with HMS OTD (filing number HU8902). In this pilot grant, we have two specific aims: first, to extend the diversity of capsule compositions, and to characterize their biophysical properties and their biocompatibility. Second, to develop applications in single cell genomics. These efforts will characterize capsules as a novel and versatile reagent.